A multienzyme bioluminescent time-resolved pyrophosphate assay
نویسندگان
چکیده
منابع مشابه
Detection of cariogenic bacteria genes by a combination of allele-specific polymerase chain reactions and a novel bioluminescent pyrophosphate assay.
We developed a novel bioluminescent assay for detection of pyrophosphate in polymerase chain reaction (PCR) product. The principle of this method is as follows: pyrophosphate released by PCR is converted to adenosine 5'-triphosphate (ATP) by pyruvate phosphate dikinase in the presence of the substrate pyruvate phosphate and the coenzyme adenosine 5'-monophosphate; subsequently, ATP concentratio...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2007
ISSN: 0003-2697
DOI: 10.1016/j.ab.2007.04.023